Q1. a)What is the function of a bacterial endospore? Hint: How easy is an endospore to destroy? Use your Handout and PowerPoint b)As a somewhat related question, is endospore formation a form of bacterial reproduction or is it doing something else for the bacteria? Explain your answer.
Q2. Why does this exercise call for use of an older culture of Bacillus? What does a positive result for a spore stain indicate about the organism? What does a negative result for the spore stain indicate about the microorganism?
Q3. List three specific functions of capsules. Use your textbook/lecture notes to help you. (Hint: two of these functions are often listed as functions of various virulence factors). Note: It is not enough to say that it protects bacteria from the immune system or that it is a virulence factor.
Q4. In the capsule stain process used in class (using nigrosin), are the bacterial cells heat-fixed to the slide? Why or why not? it is a Handout and PowerPoint
Q5. Eosin is an acidic red stain and methylene blue is a basic blue dye. What should be the result of staining a smear of encapsulated bacteria with a mixture of eosin and methylene blue?
Please note if the capsule is stained or not stained at all.
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