1) Why was this particular base pair chosen? Do you think a different non-natural base pair may have also been replicated in E. coli?
2) Why was the site (downstream of the ColE1 origin of replication) for the non-natural base pair chosen?
3) Why might growth of the E. coli be slower in the presence of IPTG? Hint: IPTG is being used to induce DNA replication.
4) What is your general thought of this research paper
Let your answers/response be detailed that show the understanding of the research paper
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