Write the reaction for nucleotide labeling. Indicate all possible byproducts that could be peaks in ion exchange chromatography purification.

Assessment for Laboratory
1. Write the reaction for nucleotide labeling. Indicate all possible byproducts that could be peaks in ion exchange chromatography purification.
2. Label the key peaks on the chromatograph and compare them with the anticipated products and reactants. Did you expect more peaks or fewer peaks? Do the peaks elute according to size or composition or both?
3. How would you test which peak contained the labeled nucleotide?
4. How would you quantify your yield?

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